7F5I
X-ray structure of Clostridium perfringens-specific amidase endolysin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-06-28 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.010, 52.870, 58.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.360 - 1.650 |
R-factor | 0.14009 |
Rwork | 0.137 |
R-free | 0.20470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lba |
RMSD bond length | 0.010 |
RMSD bond angle | 1.514 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.360 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.045 | 0.859 |
Number of reflections | 19657 | 1423 |
<I/σ(I)> | 21.1 | 2 |
Completeness [%] | 99.7 | |
Redundancy | 6.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM potassium bromide, 200 mM potassium thiocyanate, 0.1 M sodium acetate, 3 % w/v poly-gamma-glutamic acid, 5% w/v PEG 3350, pH 5.0) |