7F5I

X-ray structure of Clostridium perfringens-specific amidase endolysin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-06-28
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.010, 52.870, 58.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.360 - 1.650
R-factor	0.14009
Rwork	0.137
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lba
RMSD bond length	0.010
RMSD bond angle	1.514
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.360	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.045	0.859
Number of reflections	19657	1423
<I/σ(I)>	21.1	2
Completeness [%]	99.7
Redundancy	6.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM potassium bromide, 200 mM potassium thiocyanate, 0.1 M sodium acetate, 3 % w/v poly-gamma-glutamic acid, 5% w/v PEG 3350, pH 5.0)