7F5F

SARS-CoV-2 ORF8 S84

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	I 2 2 2
Unit cell lengths	49.521, 50.191, 80.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.546 - 1.620
R-factor	0.177312115052
Rwork	0.174
R-free	0.20501
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jtl
RMSD bond length	0.006
RMSD bond angle	0.836
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.550	1.620
High resolution limit [Å]	1.619	1.619
Number of reflections	13144	1300
<I/σ(I)>	58.9
Completeness [%]	100.0
Redundancy	12.2
CC(1/2)	1.000	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG6000, Citric acid