7F52
Crystal Structure of IBV Nsp2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.038, 192.313, 105.749 |
Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
Resolution | 49.903 - 2.559 |
R-factor | 0.2267 |
Rwork | 0.226 |
R-free | 0.25960 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.591 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.559 | 2.600 |
Rmerge | 0.010 | 0.518 |
Number of reflections | 142273 | 2796 |
<I/σ(I)> | 32.7 | |
Completeness [%] | 98.5 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 4000, MES pH 6.5, proline, urea |