7F4P

Crystal structure of SAM-bound MTA1-p2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	137.032, 137.032, 61.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.830 - 2.980
R-factor	0.2498
Rwork	0.249
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f4o
RMSD bond length	0.017
RMSD bond angle	1.595
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.020
High resolution limit [Å]	2.970	2.970
Rpim	0.023	0.284
Number of reflections	12001	675
<I/σ(I)>	28.8	2.25
Completeness [%]	99.9
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M MES, pH 6.5, 15% PEG 550 MME