7F4N
Crystal structure of SAH-bound MTA1-p1-p2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.616, 84.853, 160.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.170 - 3.120 |
R-factor | 0.2438 |
Rwork | 0.242 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7f4l |
RMSD bond length | 0.012 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rpim | 0.099 | 0.524 |
Number of reflections | 14829 | 1164 |
<I/σ(I)> | 6.2 | 1 |
Completeness [%] | 99.0 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05M HEPES, pH 7.0, 16% PEG 4000 |