7F4M

Crystal structure of SAM-bound MTA1-p1-p2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	83.517, 85.786, 162.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.230 - 3.580
R-factor	0.252
Rwork	0.251
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f4l
RMSD bond length	0.013
RMSD bond angle	1.360
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.400	3.400
Rpim	0.033	0.334
Number of reflections	13242	806
<I/σ(I)>	20.3	1.9
Completeness [%]	100.0
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.05M HEPES, pH 7.0, 16% PEG 4000