7F4A
Crystal structure of Taf14 YEATS domain in complex with H3K9bz peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 65 |
Unit cell lengths | 113.786, 113.786, 26.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.111 - 2.000 |
R-factor | 0.1774 |
Rwork | 0.176 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d7e |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.094 | 0.045 | 0.733 |
Rmeas | 0.100 | 0.048 | 0.787 |
Rpim | 0.033 | 0.016 | 0.278 |
Total number of observations | 125173 | ||
Number of reflections | 13600 | 749 | 597 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 98.7 | 99.9 | 87.5 |
Redundancy | 9.2 | 9.1 | 7.2 |
CC(1/2) | 0.997 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 48% PEG 600, 0.04M citric acid |