7F4A

Crystal structure of Taf14 YEATS domain in complex with H3K9bz peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 65
Unit cell lengths	113.786, 113.786, 26.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.111 - 2.000
R-factor	0.1774
Rwork	0.176
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d7e
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.094	0.045	0.733
Rmeas	0.100	0.048	0.787
Rpim	0.033	0.016	0.278
Total number of observations	125173
Number of reflections	13600	749	597
<I/σ(I)>	4.9
Completeness [%]	98.7	99.9	87.5
Redundancy	9.2	9.1	7.2
CC(1/2)		0.997	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	48% PEG 600, 0.04M citric acid