7F45

Structure of an Anti-CRISPR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	66.741, 66.741, 120.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.970 - 3.520
R-factor	0.2574
Rwork	0.255
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eqg
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.970	3.650
High resolution limit [Å]	3.520	3.520
Number of reflections	3096	133
<I/σ(I)>	8.25
Completeness [%]	99.9
Redundancy	22.9
CC(1/2)	1.000	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	ammonium sulfate