7F3A
Arabidopsis thaliana GH1 beta-glucosidase AtBGlu42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.690, 93.106, 60.931 |
| Unit cell angles | 90.00, 102.52, 90.00 |
Refinement procedure
| Resolution | 46.553 - 1.700 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.17980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gno |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.553 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.081 | 0.849 |
| Number of reflections | 53471 | 8612 |
| <I/σ(I)> | 15.14 | 2 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 6.76 | 5.87 |
| CC(1/2) | 0.999 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 9% (w/v) PEG 8000, 0.1 M calcium acetate, 0.05 M sodium cacodylate buffer (pH 6.5) |
