7F3A

Arabidopsis thaliana GH1 beta-glucosidase AtBGlu42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	44.690, 93.106, 60.931
Unit cell angles	90.00, 102.52, 90.00

Refinement procedure

Resolution	46.553 - 1.700
R-factor	0.1609
Rwork	0.160
R-free	0.17980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gno
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.553	1.800
High resolution limit [Å]	1.700	1.700
Rmeas	0.081	0.849
Number of reflections	53471	8612
<I/σ(I)>	15.14	2
Completeness [%]	100.0	99.7
Redundancy	6.76	5.87
CC(1/2)	0.999	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	9% (w/v) PEG 8000, 0.1 M calcium acetate, 0.05 M sodium cacodylate buffer (pH 6.5)