7F37
Crystal structure of AtaT2-AtaR2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.980 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.380, 62.210, 125.050 |
Unit cell angles | 90.00, 105.98, 90.00 |
Refinement procedure
Resolution | 45.375 - 2.896 |
R-factor | 0.2064 |
Rwork | 0.202 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fvj |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.120 | 3.000 |
High resolution limit [Å] | 2.896 | 2.900 |
Rmerge | 0.280 | 1.920 |
Number of reflections | 17558 | 1708 |
<I/σ(I)> | 7.5 | 1.4 |
Completeness [%] | 99.9 | |
Redundancy | 13.4 | |
CC(1/2) | 0.993 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Potassium formate, 20% PEG 3350 |