7F2U
FmnB complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.020, 66.489, 109.100 |
| Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
| Resolution | 27.580 - 1.984 |
| Rwork | 0.204 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7esa |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.580 | 2.055 |
| High resolution limit [Å] | 1.984 | 1.984 |
| Rmerge | 0.042 | |
| Number of reflections | 43550 | 3092 |
| <I/σ(I)> | 11.68 | |
| Completeness [%] | 91.5 | |
| Redundancy | 1.5 | |
| CC(1/2) | 0.993 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M sodium formate, 20% w/v Polyethylene glycol 3350 |
