7F2H

Crystal structure of the sensor domain of VbrK from Vibrio rotiferianus (crystal type 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	49.483, 49.727, 199.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.370 - 2.250
R-factor	0.2216
Rwork	0.220
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cus
RMSD bond length	0.008
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.066	0.603
Number of reflections	23241	1105
<I/σ(I)>	39
Completeness [%]	95.2	95.2
Redundancy	5.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 8000, Hepes