7F2H
Crystal structure of the sensor domain of VbrK from Vibrio rotiferianus (crystal type 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-08-03 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.483, 49.727, 199.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.370 - 2.250 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cus |
RMSD bond length | 0.008 |
RMSD bond angle | 1.014 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.066 | 0.603 |
Number of reflections | 23241 | 1105 |
<I/σ(I)> | 39 | |
Completeness [%] | 95.2 | 95.2 |
Redundancy | 5.6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 8000, Hepes |