7F2G

Crystal structure of the sensor domain of VbrK from Vibrio rotiferianus (crystal type 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	29.279, 75.914, 107.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.860 - 1.900
R-factor	0.1996
Rwork	0.198
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cus
RMSD bond length	0.006
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.069	0.516
Number of reflections	19318	973
<I/σ(I)>	30.9
Completeness [%]	98.2	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 8000, Hepes