7F2E

SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2020-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	102.542, 102.542, 389.677
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.844 - 3.100
R-factor	0.2691
Rwork	0.268
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wji
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.098	8.400	3.100
Rmerge	0.124	0.047	0.649
Rmeas	0.140	0.053	0.745
Rpim	0.063	0.024	0.362
Number of reflections	27752	1376	1345
<I/σ(I)>	5.4
Completeness [%]	99.9	98.9	100
Redundancy	4.8	4.8	4.2
CC(1/2)		0.998	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	0.1M phosphate citrate pH 4.5, 40% PEG 300