7F2E
SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2020-06-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 |
Unit cell lengths | 102.542, 102.542, 389.677 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.844 - 3.100 |
R-factor | 0.2691 |
Rwork | 0.268 |
R-free | 0.29750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wji |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.098 | 8.400 | 3.100 |
Rmerge | 0.124 | 0.047 | 0.649 |
Rmeas | 0.140 | 0.053 | 0.745 |
Rpim | 0.063 | 0.024 | 0.362 |
Number of reflections | 27752 | 1376 | 1345 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 4.8 | 4.8 | 4.2 |
CC(1/2) | 0.998 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1M phosphate citrate pH 4.5, 40% PEG 300 |