7F2D

Arabidopsis thaliana protease-associated domain of vacuolar-sorting receptor 1 in complex with cruciferin 1 C-terminal pentapeptide RVAAA (pH9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2018-11-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	P 1 21 1
Unit cell lengths	32.680, 60.767, 40.594
Unit cell angles	90.00, 111.62, 90.00

Refinement procedure

Resolution	37.740 - 2.450
R-factor	0.1866
Rwork	0.184
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tjx
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.740	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.037	0.086
Number of reflections	5308	587
<I/σ(I)>	23.1
Completeness [%]	96.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	289.15	0.1M Bis-Tris, 30% w/v Polyethylene glycol monomethyl ether 2000