7F2B
Crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal RNA binding domain at 2.0A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2020-09-16 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.889, 37.207, 42.845 |
| Unit cell angles | 78.69, 74.65, 65.46 |
Refinement procedure
| Resolution | 29.964 - 2.000 |
| R-factor | 0.1623 |
| Rwork | 0.157 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wji |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.964 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.141 |
| Number of reflections | 26164 | 2618 |
| <I/σ(I)> | 11.79 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 0.2 M Sodium chloride, 0.1M potassium phosphate pH 6.2, 52% v/vPEG 200 |
