7F2A

Crystal structure of Neisseria meningitidis EarP bound TDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	48.855, 125.287, 128.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.660 - 2.580
R-factor	0.2198
Rwork	0.216
R-free	0.26940
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.191
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.660	2.672
High resolution limit [Å]	2.580	2.580
Rmerge	0.109	0.757
Number of reflections	25556	2479
<I/σ(I)>	23.09	3.67
Completeness [%]	99.9
Redundancy	10.7
CC(1/2)	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Sodium cacodylate pH6.5, 1.26M Ammonium sulfate