7F26

Crystal structure of lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	297
Detector technology	PIXEL
Collection date	2021-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	78.942, 78.942, 37.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.910 - 1.700
R-factor	0.1688
Rwork	0.166
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rcm
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.470	39.470	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.175	0.118	0.433
Rmeas	0.195	0.133	0.509
Rpim	0.082	0.058	0.259
Total number of observations	64515	470	2302
Number of reflections	13336	114	690
<I/σ(I)>	7.3	10.9	3.1
Completeness [%]	97.5	94.6	95.2
Redundancy	4.8	4.1	3.3
CC(1/2)	0.980	0.974	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M citric acid and 0.2M sodium acetate were mixed in a ratio of 1:1 to form a buffer solution, and the buffer solution was mixed with 12%-16% sodium chloride in a ratio of 1:1 to form the mother liquor