7F26
Crystal structure of lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 297 |
Detector technology | PIXEL |
Collection date | 2021-05-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.942, 78.942, 37.976 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.910 - 1.700 |
R-factor | 0.1688 |
Rwork | 0.166 |
R-free | 0.19790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rcm |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.470 | 39.470 | 1.730 |
High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
Rmerge | 0.175 | 0.118 | 0.433 |
Rmeas | 0.195 | 0.133 | 0.509 |
Rpim | 0.082 | 0.058 | 0.259 |
Total number of observations | 64515 | 470 | 2302 |
Number of reflections | 13336 | 114 | 690 |
<I/σ(I)> | 7.3 | 10.9 | 3.1 |
Completeness [%] | 97.5 | 94.6 | 95.2 |
Redundancy | 4.8 | 4.1 | 3.3 |
CC(1/2) | 0.980 | 0.974 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M citric acid and 0.2M sodium acetate were mixed in a ratio of 1:1 to form a buffer solution, and the buffer solution was mixed with 12%-16% sodium chloride in a ratio of 1:1 to form the mother liquor |