7F25
Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2017-10-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.975 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 91.888, 91.888, 61.048 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.500 - 2.870 |
R-factor | 0.2361 |
Rwork | 0.233 |
R-free | 0.29580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eyg |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.970 |
High resolution limit [Å] | 2.870 | 2.870 |
Number of reflections | 7050 | 691 |
<I/σ(I)> | 20.34 | 2.31 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 5.3 | 4.9 |
CC(1/2) | 0.792 | 0.918 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15% PEG 400, 100mM sodium Salt, pH6.5 |