7F25
Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2017-10-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.888, 91.888, 61.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.500 - 2.870 |
| R-factor | 0.2361 |
| Rwork | 0.233 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyg |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.970 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Number of reflections | 7050 | 691 |
| <I/σ(I)> | 20.34 | 2.31 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.3 | 4.9 |
| CC(1/2) | 0.792 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15% PEG 400, 100mM sodium Salt, pH6.5 |
