7F22
L-lactate oxidase with pyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 |
| Unit cell lengths | 132.604, 132.604, 91.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.930 - 1.410 |
| R-factor | 0.19455 |
| Rwork | 0.193 |
| R-free | 0.21608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2du2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.839 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 1.464 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 143078 | 14714 |
| <I/σ(I)> | 16.75 | |
| Completeness [%] | 99.8 | |
| Redundancy | 17.6 | |
| CC(1/2) | 0.963 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 20 mg/ml in 50 mM sodium acetate (pH4.5) , 40% ethylene glycol |
