7F1W

X-ray crystal structure of visual arrestin complexed with inositol hexaphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-28
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	167.849, 190.465, 190.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.195 - 3.097
R-factor	0.2165
Rwork	0.215
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cf1
RMSD bond length	0.002
RMSD bond angle	0.555
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.195	3.208
High resolution limit [Å]	3.097	3.097
Rmerge	0.075	0.417
Rmeas		0.475
Rpim	0.040	0.219
Number of reflections	55228	5331
<I/σ(I)>	11.6
Completeness [%]	99.0
Redundancy	4.6
CC(1/2)	0.993	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293.15	40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000