7F1N
Beta-Glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.720, 62.210, 112.450 |
| Unit cell angles | 90.00, 98.51, 90.00 |
Refinement procedure
| Resolution | 111.213 - 2.140 |
| R-factor | 0.211842610613 |
| Rwork | 0.210 |
| R-free | 0.25252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qvb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.623 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.213 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 56997 | 3988 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 98.2 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.857 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 30% (v/v) MPD, 100mM Imidazole/Hydrochloric PH6, 5200mM Ammonium sulfate, 10% (w/v) PEG 3350 |
