7F1B

Odinarchaeota tubulin H393D mutant, in a pseudo protofilament arrangement, after GTP hydrolysis and phosphate release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.883, 94.428, 100.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.550 - 2.400
Rwork	0.208
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evh
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.117	0.605
Rmeas	0.123	0.657
Rpim	0.035	0.252
Number of reflections	15927	1616
<I/σ(I)>	13.8
Completeness [%]	99.9	99.7
Redundancy	11.3	6.7
CC(1/2)		0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	25% PEG 1500 0.1 M MMT (1 DL-malic acid: 2 MES: 2 Tris base), pH 9.0 soak: 10 mM GTP (1 h) then no GTP (14 days) 1 mM MgCl2, 0.1 M KCl