7F1A

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound 78% GTP/22% GDP 1 K+, 1 Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.416, 92.835, 102.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 1.900
Rwork	0.179
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evb
RMSD bond length	0.011
RMSD bond angle	1.143
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.620	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.123	2.337
Rmeas	0.127	2.397
Rpim	0.028	0.529
Number of reflections	31257	2053
<I/σ(I)>	14.9	1.7
Completeness [%]	100.0	100
Redundancy	19.9	20.3
CC(1/2)		0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	25% PEG 1500 0.1 M MMT (1 DL-malic acid: 2 MES: 2 Tris base), pH 9.0 Soak: 10 mM GTP, 1 mM MgCl2, 100 mM KCl (1 h)