7F18
Crystal Structure of a mutant of acid phosphatase from Pseudomonas aeruginosa (Q57H/W58P/D135R)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-02 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.542 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.822, 119.587, 190.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.630 - 3.300 |
| R-factor | 0.1948 |
| Rwork | 0.191 |
| R-free | 0.27410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f17 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.888 |
| Data reduction software | HKL-2000 |
| Data scaling software | SAINT (V8.38A) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.630 | 3.520 |
| High resolution limit [Å] | 3.189 | 3.260 |
| Number of reflections | 13485 | 855 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 0.1M BIS-TRIS pH 6.5, 50% v/v Polypropylene glycol P 400 |
