7F15
A SARS-CoV-2 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.995, 74.995, 213.113 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.520 - 2.650 |
R-factor | 0.2053 |
Rwork | 0.201 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7f0x |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.113 | 0.953 |
Number of reflections | 34387 | 1024 |
<I/σ(I)> | 25.8 | 2.8 |
Completeness [%] | 99.9 | |
Redundancy | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.1M Sodium malonate pH 4.0, 12% w/v Polyethylene glycol 3350 |