7F13
Crystal structure of isomerase Dcr3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 104.999, 104.999, 139.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.160 - 2.150 |
| R-factor | 0.2333 |
| Rwork | 0.230 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f0o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.050 | 0.697 |
| Number of reflections | 25477 | 2161 |
| <I/σ(I)> | 39 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.9 | 22.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl (pH8.5), 15% w/v PEG8000, 250 mM KF |
