7F12
A SARS-CoV-2 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-10-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.460, 75.460, 184.135 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.740 - 3.150 |
R-factor | 0.2 |
Rwork | 0.195 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7f0x |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.121 | 1.236 |
Number of reflections | 19115 | 533 |
<I/σ(I)> | 21.5 | 1.86 |
Completeness [%] | 99.7 | |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.2M Sodium phosphate monobasic monohydrate pH 4.7, 20% w/v Polyethylene glycol 3350 |