7F0X
A SARS-CoV-2 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.369, 75.369, 186.632 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.030 - 2.800 |
R-factor | 0.2261 |
Rwork | 0.221 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7deu |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.660 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 15742 | 2243 |
<I/σ(I)> | 7.9 | |
Completeness [%] | 99.6 | |
Redundancy | 5.2 | |
CC(1/2) | 0.970 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.2M Potassium phosphate monobasic pH4.8, 20% w/v Polyethylene glycol 3350 |