7F0K
Crystal structure of Kmp11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-05-14 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.899, 38.544, 142.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.320 - 3.500 |
R-factor | 0.2594 |
Rwork | 0.257 |
R-free | 0.29840 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.334 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.625 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 2583 | 236 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.2 | |
Redundancy | 3.1 | |
CC(1/2) | 0.800 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 280 | Ammonium Sulfate, Polyethylene glycol 4000 |