7F0H

Structural and functional characterization of bovine G1P[5] rotavirus VP8* protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	113
Detector technology	CCD
Collection date	2019-04-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.03923
Spacegroup name	I 21 21 21
Unit cell lengths	97.753, 102.364, 137.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.806 - 1.695
R-factor	0.1678
Rwork	0.167
R-free	0.18750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jdb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.695	1.700
Rmerge	0.094	0.874
Number of reflections	76163
<I/σ(I)>	24.004	2.409
Completeness [%]	98.5
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.5 M ammonium sulfate, 0.1 M tri-sodium citrate dihydrate pH 5.6, 1.0 M lithium sulfate monohydrate