7F0B
Crystal structure of capreomycin phosphotransferase in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9732 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 91.833, 91.833, 120.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.250 - 2.140 |
| R-factor | 0.2093 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f0a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.735 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 29140 | 2835 |
| <I/σ(I)> | 55.9 | 2.32 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.6 | |
| CC(1/2) | 0.984 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.4 M sodium malonate pH 7.0, 0.1 M Bis-Tris propane pH 7.0, 0.1 M calcium chloride dihydrate |
