7F01

Structure of a triple-helix region of human collagen type XVII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-05-31
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	20.320, 21.260, 67.600
Unit cell angles	90.00, 90.38, 90.00

Refinement procedure

Resolution	22.530 - 1.630
R-factor	0.2195
Rwork	0.217
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wuh
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	1.660
High resolution limit [Å]	1.628	1.630
Rmerge	0.160	0.416
Rmeas	0.174	0.451
Rpim	0.067	0.171
Number of reflections	7196	335
<I/σ(I)>	7.5
Completeness [%]	96.0	93
Redundancy	6.5	6.5
CC(1/2)	0.991	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	1.0 M Potassium sodium tartrate, 0.1 M CHES/ Sodium hydroxide pH 9.5, 0.2 M Lithium sulfate