7EZY
anti-CRISPR-associated Aca2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 125 |
| Detector technology | PIXEL |
| Collection date | 2021-03-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.130, 64.130, 135.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.980 - 1.920 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a4a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.980 | 1.989 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmeas | 0.138 | |
| Number of reflections | 22344 | 2162 |
| <I/σ(I)> | 19.56 | |
| Completeness [%] | 99.8 | |
| Redundancy | 25.8 | |
| CC(1/2) | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 25% PEG 1000, 0.1M Tris base pH 8.9 |
