7EZO

GH10 domain of bifunctional endoxylanase and arabinofuranosidase of Bi0569

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	70.199, 70.199, 106.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.554 - 2.800
R-factor	0.250233767899
Rwork	0.248
R-free	0.28528
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	CNS
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.560	2.500
High resolution limit [Å]	2.500	2.500
Number of reflections	17923	10
<I/σ(I)>	3
Completeness [%]	100.0
Redundancy	2.4
CC(1/2)	1.000	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	PEG3350, K2HPO4 pH8.2, Na2HPO4 pH6.9