7EZN
Crystal structure of CnYvh1 complex with vanadate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.194, 66.194, 120.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.100 - 1.800 |
R-factor | 0.2008 |
Rwork | 0.200 |
R-free | 0.20890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ki9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.545 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.100 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.011 | 0.213 |
Number of reflections | 24183 | 1709 |
<I/σ(I)> | 33.71 | |
Completeness [%] | 94.6 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.16 M Magnesium chloride, 0.08 M Tris-HCl pH 8.5, 24 % (w/v) PEG 4000, 20 % glycerol |