7EZE

Thorarchaeota Rab GTP-gamma-S/Mg2+ (soak)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-16
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	69.133, 69.133, 66.927
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 1.950
Rwork	0.166
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ezb
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.087	0.820
Rmeas	0.096	0.964
Rpim	0.041	0.498
Number of reflections	25510	1304
<I/σ(I)>	16.7	1.2
Completeness [%]	97.8
Redundancy	5.4
CC(1/2)		0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES, pH 5.5, 250 mM NaCl, 13% PEG 8000, 5 mM GTP soaked with 10 mM GTP gamma-S, 15 mM MgCl2 (1 h)