7EZD

Thorarchaeota Rab co-crystallized with GTP gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	68.656, 68.656, 66.579
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.350 - 1.760
Rwork	0.164
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ezb
RMSD bond length	0.004
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.350	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.078	1.123
Rmeas	0.087
Rpim	0.038	0.549
Number of reflections	34996	10206
<I/σ(I)>	11	1.5
Completeness [%]	99.9
Redundancy	5.2
CC(1/2)		0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES, pH 5.5, 250 mM NaCl, 13% PEG 8000, 5 mM GTP gamma-S