7EYW
Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with terretonin C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-27 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.07 |
Spacegroup name | P 1 |
Unit cell lengths | 49.651, 72.281, 78.455 |
Unit cell angles | 81.93, 84.88, 70.35 |
Refinement procedure
Resolution | 48.240 - 2.100 |
R-factor | 0.2073 |
Rwork | 0.206 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7eyr |
RMSD bond length | 0.002 |
RMSD bond angle | 0.567 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.240 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.065 | 0.632 |
Number of reflections | 57878 | 9566 |
<I/σ(I)> | 7.3 | 1.8 |
Completeness [%] | 97.6 | 96.2 |
Redundancy | 2.1 | 2.1 |
CC(1/2) | 0.996 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.27 M magnesium chloride, 20% w/v PEG 3350, 0.2 M sodium chloride, 1.5% trimethylamine N-oxide dihydrate |