7EYT
Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-26 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 50.054, 72.557, 78.826 |
Unit cell angles | 81.74, 84.19, 70.23 |
Refinement procedure
Resolution | 48.420 - 2.100 |
R-factor | 0.2378 |
Rwork | 0.236 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7eyr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.595 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.420 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.138 | 0.456 |
Number of reflections | 56771 | 4484 |
<I/σ(I)> | 5.7 | 2.5 |
Completeness [%] | 94.3 | 95.1 |
Redundancy | 3.3 | 3.6 |
CC(1/2) | 0.986 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.27 M magnesium chloride, 16% w/v PEG 3350, 1.5% trimethylamine N-oxide dihydrate |