7EYP

Crystal structure of Pseudomonas aeruginosa ppnP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	61.599, 61.599, 96.482
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.181
Rwork	0.179
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eyj
RMSD bond length	0.008
RMSD bond angle	1.015
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.103	0.740
Rmeas	0.105	0.760
Rpim	0.024	0.171
Number of reflections	33692	3228
<I/σ(I)>	30.875	6.75
Completeness [%]	97.6	96.3
Redundancy	19.1	19.1
CC(1/2)	0.989	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate