7EYO
Crystal structure of leech hyaluronidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.514, 69.474, 86.739 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 43.320 - 1.850 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5la4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.077 | 0.063 | 0.496 |
| Rmeas | 0.084 | 0.068 | 0.540 |
| Rpim | 0.032 | 0.027 | 0.210 |
| Total number of observations | 295605 | ||
| Number of reflections | 43983 | 4515 | 4103 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 99.2 | 99.8 | 92.9 |
| Redundancy | 6.7 | 6.5 | 6.3 |
| CC(1/2) | 0.994 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | trisodium citrate, ED-2001 |
