7EYK
Crystal structure of Escherichia coli ppnP-Selenomethionine derived
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 62 |
Unit cell lengths | 59.658, 59.658, 52.652 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.380 |
R-factor | 0.1515 |
Rwork | 0.151 |
R-free | 0.16880 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (v1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.093 | 0.505 |
Rmeas | 0.079 | 0.518 |
Rpim | 0.018 | 0.116 |
Number of reflections | 21812 | 2162 |
<I/σ(I)> | 36.41 | 7 |
Completeness [%] | 100.0 | |
Redundancy | 19.8 | |
CC(1/2) | 0.960 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.26 M Ammonium sulfate (dibasic), 0.1M Sodium cacodylate/Hydrochloric acid pH 6.5 |