7EYJ
Crystal structure of Escherichia coli ppnP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 62 |
Unit cell lengths | 59.892, 59.892, 52.145 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.380 |
R-factor | 0.1534 |
Rwork | 0.152 |
R-free | 0.17800 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.867 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (v1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.084 | 0.196 |
Rpim | 0.019 | 0.046 |
Number of reflections | 21996 | 2189 |
<I/σ(I)> | 37.3 | 18.7 |
Completeness [%] | 99.9 | |
Redundancy | 19.9 | |
CC(1/2) | 0.987 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0 M Ammonium sulfate (dibasic),0.1M CAPS/Sodium hydroxide pH 10.5, 0.2M Lithium sulfate |