7EY1
Bifunctional xylosidase/glucosidase LXYL with intermediate substrate xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 228.508, 88.174, 218.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 218.500 - 2.460 |
| Rwork | 0.196 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kj0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 218.500 | 2.500 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.144 | 0.537 |
| Number of reflections | 158265 | 7844 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 15% PEG 3350, 0.1M Hepes pH 7.5, 0.2% MgCl2 |
