7EXV
GH127 beta-L-arabinofuranosidase HypBA1 covalently complexed with beta-L-arabinofuranoylamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-26 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.547, 77.547, 253.529 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.140 - 2.600 |
R-factor | 0.2541 |
Rwork | 0.251 |
R-free | 0.30960 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3wkx |
RMSD bond length | 0.005 |
RMSD bond angle | 1.421 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.140 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.140 | 1.306 |
Rpim | 0.045 | 0.412 |
Number of reflections | 28225 | 3353 |
<I/σ(I)> | 9 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.7 | 10.9 |
CC(1/2) | 0.998 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.7 M sodium citrate, 0.1 M MES-NaOH (pH 6.5) |