7EXP

Crystal structure of zebrafish TRAP1 with AMPPNP and MitoQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-07
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	179.339, 96.522, 125.615
Unit cell angles	90.00, 134.39, 90.00

Refinement procedure

Resolution	31.205 - 2.297
R-factor	0.1967
Rwork	0.195
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ipe
RMSD bond length	0.008
RMSD bond angle	1.404
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.297	2.300
Rmerge	0.088	0.424
Number of reflections	67378	3161
<I/σ(I)>	21
Completeness [%]	99.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	18% PEG3350, 0.2M Sodium malonate pH 6.5, 30uM hexaaminecobalt(III) chloride