7EXM

The N-terminal crystal structure of SARS-CoV-2 NSP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	57.926, 159.603, 63.552
Unit cell angles	90.00, 91.19, 90.00

Refinement procedure

Resolution	42.400 - 1.960
Rwork	0.200
R-free	0.22560
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.285
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	1.960	1.960
Number of reflections	89477	4474
<I/σ(I)>	17.9
Completeness [%]	93.0
Redundancy	6
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG