7EXI

Crystal structure of the BTB domain human Keap1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 65 2 2
Unit cell lengths	42.976, 42.976, 266.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.620 - 1.820
R-factor	0.2108
Rwork	0.209
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cxi
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.820	4.970	1.830
Rmerge	0.090	0.059	1.441
Rmeas	0.093	0.061	1.493
Rpim	0.023	0.015	0.386
Number of reflections	13888	828	696
<I/σ(I)>	5.9
Completeness [%]	97.8	92.9	100
Redundancy	15.5	14.9	14.6
CC(1/2)		0.999	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, Li Acetate, TCEP