7EXH
Crystal structure of D383A mutant from Arabidopsis thaliana complexed with Galactinol.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2018-04-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.520, 103.649, 182.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.220 - 2.630 |
R-factor | 0.1858 |
Rwork | 0.183 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7exg |
RMSD bond length | 0.014 |
RMSD bond angle | 1.783 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 91.220 | 91.220 | 2.780 |
High resolution limit [Å] | 2.630 | 7.650 | 2.630 |
Rmerge | 0.098 | 0.050 | 0.550 |
Rmeas | 0.118 | 0.060 | 0.662 |
Total number of observations | 326510 | ||
Number of reflections | 105231 | 4147 | 17038 |
<I/σ(I)> | 9.28 | 20.27 | 2.33 |
Completeness [%] | 98.6 | 96.2 | 99.1 |
Redundancy | 3.103 | 3.154 | 3.159 |
CC(1/2) | 0.993 | 0.994 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.8 | 291.5 | 0.1 M Tris, PEG 2000, PGA |